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Biotin (Vitamin B7)
Quest Diagnostics1 test optionFrom $554.17
Biotin (Vitamin B7)
Quest DiagnosticsCatalog information reviewed: August 24, 2026 by the Sunridge Medical clinical team.
What this laboratory test evaluates
DetailsBiotin (Vitamin B7) – Biotin, vitamin B7, or vitamin H, is a water-soluble vitamin. The vitamin plays a role in transferring carbon dioxide in the metabolism of fat, carbohydrate, and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions, including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone through the action of biotinyl-transferase.
Sometimes the vitamin is used in weight reduction programs. It may be prescribed as a supplement for diabetic patients due to its role in carbohydrate metabolism.
Biotin is commonly found in vitamin B complex and many food sources, such as milk, yeast, egg yolk, cereal, and mushrooms. The reference daily intake [RDI of 101.9(c) (8) (IV)] for vitamin B7 is 300 micrograms. Deficiency in the vitamin may result in seborrheic dermatitis, alopecia, myalgia, hyperesthesia, and conjunctivitis.
Disorders of biotin metabolism can be acquired or congenital. Biotinidase and holocarboxylase synthethase deficiency are the two better-known forms of disorders. The lack of biotin-dependent pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and acetyl-CoA carboxylase can lead to the life-threatening disorder of multiple carboxylase deficiency. Treatment involves a daily dose of approximately 10 mg biotin/day. Irreversible mental or neurological abnormalities may result from delayed clinical intervention.
What type of sample is required for this test?2 mL frozen serum – protect from light
Test ResourcesFor more information, please consult your healthcare provider.
Additional test information
Biotin (Vitamin B7) – Biotin, vitamin B7, or vitamin H, is a water-soluble vitamin that plays a role in transferring carbon dioxide in the metabolism of fat, carbohydrate, and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions, including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone through the action of biotinyl-transferase.
Collection and processing
- Sample type: Serum
- Fasting: No
- Estimated laboratory processing: Results should be available in 2 – 5 business days after the sample is collected
How Sunridge uses laboratory information
Sunridge considers the medical question, symptoms, history, medications, prior results, and whether the finding could change care before recommending testing. A laboratory result is not interpreted in isolation and does not establish a diagnosis by itself.
Before requesting this test
Availability, collection requirements, eligibility, and processing estimates can change. Sunridge reviews each request and, when appropriate, creates the order through Fullscript. Follow the current instructions supplied with the approved order.
Common questions about this laboratory test
- What sample does this test require?
- The listed sample type is Serum. Confirm current collection instructions in the approved order.
- Does this test require fasting?
- The current catalog record lists fasting as: No. Follow the instructions supplied with the approved order.
- How long does laboratory processing usually take?
- Results should be available in 2 – 5 business days after the sample is collected. Timing can change after collection or if a laboratory requires additional review.
About this test
DetailsBiotin (Vitamin B7) - Biotin, vitamin B7, or vitamin H, is a water-soluble vitamin. The vitamin plays a role in transferring carbon dioxide in the metabolism of fat, carbohydrate, and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions, including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone through the action of biotinyl-transferase.
Sometimes the vitamin is used in weight reduction programs. It may be prescribed as a supplement for diabetic patients due to its role in carbohydrate metabolism.
Biotin is commonly found in vitamin B complex and many food sources, such as milk, yeast, egg yolk, cereal, and mushrooms. The reference daily intake [RDI of 101.9(c) (8) (IV)] for vitamin B7 is 300 micrograms. Deficiency in the vitamin may result in seborrheic dermatitis, alopecia, myalgia, hyperesthesia, and conjunctivitis.
Disorders of biotin metabolism can be acquired or congenital. Biotinidase and holocarboxylase synthethase deficiency are the two better-known forms of disorders. The lack of biotin-dependent pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and acetyl-CoA carboxylase can lead to the life-threatening disorder of multiple carboxylase deficiency. Treatment involves a daily dose of approximately 10 mg biotin/day. Irreversible mental or neurological abnormalities may result from delayed clinical intervention.
What type of sample is required for this test?2 mL frozen serum - protect from light
Test ResourcesFor more information, please consult your healthcare provider.
Additional details
Biotin (Vitamin B7) - Biotin, vitamin B7, or vitamin H, is a water-soluble vitamin that plays a role in transferring carbon dioxide in the metabolism of fat, carbohydrate, and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions, including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone through the action of biotinyl-transferase.
Highlighted health areas
- Nutrition
Biomarkers and report focus
The exact analytes and report format can change. Confirm the current contents in the Fullscript order before payment.
- Biotin (Vitamin B7)