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RPR (Diagnosis) with Reflex to Titer and Treponema pallidum Antibody, IA
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RPR (Diagnosis) with Reflex to Titer and Treponema pallidum Antibody, IA
Quest DiagnosticsCatalog information reviewed: August 24, 2026 by the Sunridge Medical clinical team.
What this laboratory test evaluates
DetailsRPR (Diagnosis) with Reflex to Titer and Treponema pallidum Antibody, IA – This test is used to aid in the diagnosis of syphilis using the traditional algorithm. The algorithm starts with RPR, a nontreponemal (lipoidal antigen) serologic test, and if reactive will reflex to titer. Reactive RPR samples will be further tested with the Treponema pallidum Antibody immunoassay, a treponemal serologic test.
Testing can be performed for individuals suspected of syphilis based on signs and symptoms or for screening asymptomatic individuals. Screening is recommended in certain populations at increased risk for infection and pregnant women.
False positive results with nontreponemal (lipoidal antigen) test results may occur due to wide range of biological conditions (e.g., autoimmune disease, cancer, infectious diseases, old age, and pregnancy). False negatives can occur in persons with recent exposure. Persons with previously treated syphilis will remain positive using a treponemal test. Therefore, a single type of serological test is insufficient for diagnosis and positive result using a nontreponemal (lipoidal antigen) test should be confirmed by a treponemal specific assay using the traditional algorithm.
Test Resources• Test FAQ: Syphilis (Treponema pallidum)
• Algorithm: Traditional Algorithm for Serologic Diagnosis of Syphilis
• Clinical Focus: Syphilis: Laboratory Support for Screening, Diagnosis, and Monitoring
Additional test information
RPR (Diagnosis) with Reflex to Titer and Treponema pallidum Antibody, IA – This test is used to aid in the diagnosis of syphilis using the traditional algorithm. Testing can be performed for individuals suspected of syphilis based on signs and symptoms or for screening asymptomatic individuals. Screening is recommended in certain populations at increased risk for infection and pregnant women.
Collection and processing
- Sample type: Serum
- Fasting: No
- Estimated laboratory processing: Results should be available in 2 – 5 business days after the sample is collected
How Sunridge uses laboratory information
Sunridge considers the medical question, symptoms, history, medications, prior results, and whether the finding could change care before recommending testing. A laboratory result is not interpreted in isolation and does not establish a diagnosis by itself.
Before requesting this test
Availability, collection requirements, eligibility, and processing estimates can change. Sunridge reviews each request and, when appropriate, creates the order through Fullscript. Follow the current instructions supplied with the approved order.
Common questions about this laboratory test
- What sample does this test require?
- The listed sample type is Serum. Confirm current collection instructions in the approved order.
- Does this test require fasting?
- The current catalog record lists fasting as: No. Follow the instructions supplied with the approved order.
- How long does laboratory processing usually take?
- Results should be available in 2 – 5 business days after the sample is collected. Timing can change after collection or if a laboratory requires additional review.
About this test
DetailsRPR (Diagnosis) with Reflex to Titer and Treponema pallidum Antibody, IA - This test is used to aid in the diagnosis of syphilis using the traditional algorithm. The algorithm starts with RPR, a nontreponemal (lipoidal antigen) serologic test, and if reactive will reflex to titer. Reactive RPR samples will be further tested with the Treponema pallidum Antibody immunoassay, a treponemal serologic test.
Testing can be performed for individuals suspected of syphilis based on signs and symptoms or for screening asymptomatic individuals. Screening is recommended in certain populations at increased risk for infection and pregnant women.
False positive results with nontreponemal (lipoidal antigen) test results may occur due to wide range of biological conditions (e.g., autoimmune disease, cancer, infectious diseases, old age, and pregnancy). False negatives can occur in persons with recent exposure. Persons with previously treated syphilis will remain positive using a treponemal test. Therefore, a single type of serological test is insufficient for diagnosis and positive result using a nontreponemal (lipoidal antigen) test should be confirmed by a treponemal specific assay using the traditional algorithm.
Test Resources• Test FAQ: Syphilis (Treponema pallidum)
• Algorithm: Traditional Algorithm for Serologic Diagnosis of Syphilis
• Clinical Focus: Syphilis: Laboratory Support for Screening, Diagnosis, and Monitoring
Additional details
RPR (Diagnosis) with Reflex to Titer and Treponema pallidum Antibody, IA - This test is used to aid in the diagnosis of syphilis using the traditional algorithm. Testing can be performed for individuals suspected of syphilis based on signs and symptoms or for screening asymptomatic individuals. Screening is recommended in certain populations at increased risk for infection and pregnant women.
Highlighted health areas
- Infectious Disease
Biomarkers and report focus
The exact analytes and report format can change. Confirm the current contents in the Fullscript order before payment.
- RPR (Reagin Ab)
- Treponema Pallidum Ab
- RPR Titer